Florentina of Cartagena was a sixth-century nun who lived in Hispania during the Visigothic period. Born in Cartagena, she belonged to a prominent family of Christian leaders, being the sister of three Iberian bishops, including Leander and Isidore of Seville. Under the spiritual guidance of her brother Leander, she dedicated herself to an ascetic lifestyle and established a religious community for virgins. She eventually served as an abbess, leading her community according to the monastic rules established by her brother.